By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)
Animal mobile know-how is a turning out to be self-discipline of mobilephone biology which goals not just to appreciate constructions, capabilities and behaviors of differentiated animal cells, but additionally to envision their talents for use for commercial and scientific reasons. The aim of animal telephone know-how comprises the clonal growth of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of scientific and pharmaceutical importantance, and the appliance of animal cells to gene treatment, synthetic organs and the creation of sensible meals. This quantity offers the readers an entire evaluate of the current cutting-edge and may be precious for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, relatively phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines regarding animal cellphone culture.
Read or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF
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Extra resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006
1% concentration. 2 Cell Line and Culture Conditions A mouse hybridoma 2E3-O cell line was grown in serum-free medium ASF103 (Ajinomoto, Japan) at 37°C in a humidified atmosphere of 5% CO2 in air . 3 Cell Proliferation Assay To assay the effect of sericin on proliferation under conditions of growth factor starvation, 2E3-O cells were seeded at a density of 13 × 105 cells in 6 ml ASF104 medium (Ajinomoto) with reduced growth supplements. 1% sericin. Viable cell density was measured by counting using the trypan blue dye exclusion method, under phase-contract microscopy at several time points for 37 h.
104 cells/ ml] Viable cell conc. [×104 cells / ml] a T. Sogo et al. 1 1 60 10 50 40 20 0 0 1 2 3 4 5 6 Culture period [day] Viable cell conc. 1 1 10 50 80 60 40 20 0 8 HEL conc. (µg/ml) 0 1 2 3 4 5 6 7 Culture period [day] 8 9 BSA-FL conc. 1 80 1 5 60 10 40 20 0 0 1 2 3 4 Culture period [day] 5 Fig. 3 Cell growth curves of transfected CTLL-2 cells. HEL selected CT/HbLg (a), CT/HgLb (b) were cultured with various concentrations of HEL, whereas BSA-FL-selected CT/SbSg (c) were cultured with BSA-FL.
Sericin successfully improved cell survival under heat stress. 1 Effect of Sericin on Caspase Activity Sericin suppressed cell death, therefore, we examined whether sericin inhibited caspase activity. Figure 2 shows that activity of caspase3/7, an executioner for inducing apoptosis, was decreased by sericin treatment. In contrast, caspase9 activity Effects of Sericin on Promoting Proliferation and Inhibiting Apoptosis 35 was not decreased by sericin (data not shown). These results suggest that sericin suppressed apoptosis via suppression of caspase3/7.
Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 by Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)